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EMBO Member

Robert Kaptein

University, Utrecht | Netherlands

EMBO 1991 | FelC 92–95 | YipC 12–16

NMR studies of protein-DNA interaction

Our research has dealt with the characterization of protein-DNA interactions in transcription regulation and DNA repair using the methods of NMR spectroscopy. For the lac repressor of E.coli we have determined the structure of both specific and non-specific DNA complexes. We have elucidated the mode of DNA sliding, and the allosteric binding mechanism of the repressor.

Keywords: Protein structure / protein-DNA interaction / NMR spectroscopy / nuclear spin hyperpolarization / CIDNP

Subject area(s): Proteins & Biochemistry | Structural Biology & Biophysics

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