EMBO Member

Ruth Sperling

Hebrew University, Jerusalem | Israel

EMBO 1994

The supraspliceosome: the in vivo pre-mRNA processing machine

We showed that pre-mRNA processing including splicing occurs in the cell nucleus within a huge (21 MDa), dynamic RNP machine – the supraspliceosome – composed of four native spliceosomes, connected by the pre-mRNA. Studying its structure and function, we solved the structure of the native spliceosome by cryo-EM at 20Å resolution, and showed that this machine regulates splicing, alternative splicing, and processing of intronic small non-coding RNAs.

Keywords: RNA processing / protein-RNA interaction / RNP structure & function / constitutive & alternative splicing / small non coding RNA

Subject area(s): Proteins & Biochemistry | RNA | Structural Biology & Biophysics